ANTIMICROBIAL ACTIVITY OF CANNABIDIOL ON BACTERIA CAPABLE OF FORMING BIOFILM

Abstract

Some bacterial infections have been in the past years and till date a very tough disease to cure and get rid of with the available antibiotics that can be found in hospitals and pharmacies. This has led to scientist to experiment using many different plant materials to test against such infections to find a total cure of such diseases. One of the plants that have this potential is Cannabis sativa. One of the bioactive compounds of C. sativa that is suspected to have the potential to treat stubborn bacterial infection is Cannabidiol and also called CBD. The objective of this research is to get pure CBD and use it against stubborn bacterial infections caused by Staphylococcus aureus to see if it will be able to treat it. S. aureus were isolated from clinical samples from open wounds and skin infections. The pure CBD was used against the bacteria using disc diffusion method to check if it is susceptible, resistant or intermediate after confirming biofilm formation by the bacterial through 96-well method. Discs impregnated with Penicillin, Ampicillin and distilled water were used as the control. The mean average for Penicillin, Ampicillin and Cannabidiol on ten positive samples were 09mm, 10mm and 18mm, respectively. Using Kirby-Bauer Antibiotic Sensitivity standardized chart, Penicillin and Ampicillin showed that S. aureus are resistant to them. While cannabidiol has no standard yet, but 18mm was the mean zone of inhibition recorded now. This could mean that, S. aureus are resistant, susceptible or intermediate using pure Cannabidiol as the antibiotic when a standard has been agreed for CBD as a possible antibiotic.