INVITRO ANTIOXIDANT AND ANTI-INFLAMMATORY ACTIVITIES OF Chrysanthenium indicum EXTRACT

Abstract

The present investigation was aimed to justify the scientific basis in traditional use of Chrysanthemum indicum as radicals scavenging herbal supplement. In-vitro antioxidant activities were evaluated by Reducing power method, Superoxide scavenging method and DPPH radical scavenging method. In-vitro anti-inflammatory activities were evaluated using albumin denaturation and membrane stabilizing method. Both methanol and aqueous extracts of Chrysanthenium indicum showed significant percentage inhibition of protein denaturation for methanol (61.87) and aqueous (51.14) extract compare to Diclofenac sodium (89.27); and hemolysis methanol (31.86) and aqueous (28.14) extract compare to Diclofenac sodium (51.27); at 0.10mg/ml. The extracts also exhibited antioxidant activities against ascorbic acid as a standard in which DPPH method for methanol (56.6) and aqueous (43.7) extract compare to ascorbic acid (85.7); super oxide radicals methanol (62.9) and aqueous (61.3) extract compare to ascorbic acid (85.8); and reducing power activity methanol (72.3) and aqueous (64.7) extract compare to ascorbic acid (87.3) at 100μg/ml The maximum 96% inhibition by Gallic acid equivalents and Quercetin 81%. Total phenolic content equivalent flavonoids content in Methanol extracts were estimated that was three fold (phenolic content) is two fold (flavonoids) more than that in aqueous extract. These observations established the influence of solvent on both antioxidant and Invitro anti-inflammatory activities was obvious. Chrysanthemum indicum activities against inflammation and oxidative stress could be due to cyclooxygenase enzyme inhibition and free radical scavenging activities of the extract.